Coding
fiatluxD

Part:BBa_K4239002:Design

Designed by: Guillaume FULCONIS   Group: iGEM22_INSA_Lyon1   (2022-10-05)
Revision as of 11:32, 11 October 2022 by Gfulconis (Talk | contribs)


Enhanced luciferase subunits fiatluxD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Igem restriction site Xba1 was removed into the iluxD part, to create fiatluxD. An Adenine was replace by a Guanine. The mutation does not change the protein sequence.

iluxD did not have any mutation compare to luxD, according to Gregor et al.’s study in 2018.

Source

The source of fiatluxD is synthesis. This part was synthetized from the nucleotidique sequence according to Gregor et al.’s study in 2018.

This part was synthetized directly with fiatluxC and the promoter BBa_J23117.

References

Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359.