Composite

Part:BBa_K4238007

Designed by: Keita Hoshino   Group: iGEM22_UTokyo   (2022-10-09)
Revision as of 13:22, 12 October 2022 by Asako (Talk | contribs)


ADH1p+loxP+mCherry+CYC1t+loxP+mNeonGreen+ADH1t

This part was created to assay the recombination by the Cre-loxP system in yeast.

When Cre is not present in the cell, mCherry (red fluorescent protein) flanked by loxP is expressed, while mNeonGreen (green fluorescent protein) in the back is not expressed because of the CYC1 terminator. On the other hand, if Cre is present in the cell, only mNeonGreen is expressed because the mCherry sequence is cut out. Therefore, the recombination timescale and other parameters can be evaluated by observing the fluorescence color under a microscope.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None