Composite

Part:BBa_K200009

Designed by: David Roche, Royah Vaezi   Group: iGEM09_Imperial College London   (2009-08-12)
Revision as of 13:27, 29 September 2009 by Droche (Talk | contribs) (→‎Usage and Biology)

DpnII Composite

This sequence codes for the restriction enzyme DpnII.

This is a Type II restriction enzyme that recognises the sequence GATC. Its activity can be blocked by dam methylation.

The working temperature is 37°C.

As part of the Imperial 2009 iGEM E.ncapsulator project, TaqI was used along with DpnII to cut up the genetic material within the cell, completing the cell death module.


Usage and Biology

DpnII is a 4 base cutter, leaving a 4 base overhang.


The cutting site is as shown:

II09 DpnII.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[http://www.neb.com/nebecomm/products/productR0543.asp Information from NEB]



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Parameters
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