Part:BBa_K200005

OtsA: Part 1 of 2 for trehalose producing enzymes.

Sequence codes for trehalose-6-phosphate synthase enzyme. This enzyme is the first of two required in the conversion of glucose to trehalose.
This enzyme catalyses the following reaction:
UDP-glucose + D-glucose 6-phosphate -> UDP + alpha,alpha-trehalose 6-phosphate

[http://en.wikipedia.org/wiki/Trehalose Trehalose] is a disaccharide formed from two glucose molecules. Throughout nature, trehalose is associated with resistance to dessication and cold shock otsa1, and is naturally produced in Escherichia Coli. We hope that by upregulating the trehalose production pathways in E.coli we can increase trehalose concentrations within our cell, thereby conferring some resistance to protein degredation in our system. This would allow easy transport and storage of the final product.

Usage and Biology

Transcription of OtsA gene is activated by osmotic stress in E. coli.otsa1

mRNA of OtsA is more stable at 16°C, therefore, it is a cold inducible mRNA.otsa2

OtsA mutation will block the synthesis of the trehalose-6-phosphase synthase, which is the enzyme that converts glucose-6-phosphate to trehalose-6-phosphate. otsa2

The gene was used alongside OtsB by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] team as part of the storage protection mechanism. It was also previously used by the UC Berkeley 2007 iGEM team as part of the [http://parts.mit.edu/igem07/index.php/Berkeley_UC Bactoblood] project to enable freeze-drying of the system.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 383
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

<biblio>

1. otsa1 pmid=12105274
2. otsa2 pmid=1310094

</biblio>