Coding

Part:BBa_K4339009

Designed by: Chloe Singleton   Group: iGEM22_Exeter   (2022-10-09)
Revision as of 08:57, 9 October 2022 by ChloeS (Talk | contribs)


MaSp2 6Rep

A major ampullate spidroin 2 (MaSp2) based protein consisting of six repetitive regions from the black widow spider Latrodectus hesperus. Each repetitive region begins with a glycine rich region and ends with between seven and nine alanines.


Usage and Biology

Sequence and Features

The sequence originates from the black widow spider Latrodectus hesperus (Ayoub et al. 2007 and You et al. 2018).


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 193
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Ayoub, N.A. et al. (2007) ‘Blueprint for a High-Performance Biomaterial: Full-Length Spider Dragline Silk Genes’, PLoS ONE, 2(6). Available at: https://doi.org/10.1371/journal.pone.0000514.

You, Z. et al. (2018) ‘Extraordinary Mechanical Properties of Composite Silk Through Hereditable Transgenic Silkworm Expressing Recombinant Major Ampullate Spidroin’, Scientific Reports, 8(1), pp. 1–14. Available at: https://doi.org/10.1038/s41598-018-34150-y.


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