Regulatory

Part:BBa_K4343124

Designed by: Xiaolu Sun   Group: iGEM22_NNU-China   (2022-10-09)
Revision as of 05:44, 9 October 2022 by Milulu (Talk | contribs)

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YAT

Derived from Y. lipolytica, YATin promotor is a specific DNA sequence upstream of the 5' end of a structural gene that can be specifically recognized and bound by RNAP. This promoter has been used to control the expression of the required enzyme genes in Y. lipolytica to iprove the production of omage-3 polyunsaturated fatty acids.

Reference: Xue, Z., Sharpe, P. L., Hong, S. P., Yadav, N. S., Xie, D., Short, D. R., ... & Zhu, Q. (2013). Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica. Nature biotechnology, 31(8), 734-740.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 604
    Illegal NheI site found at 959
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 828
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1153


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Categories
Parameters
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