Measurement

Part:BBa_K4204016

Designed by: Jianfei Song   Group: iGEM22_BNDS_China   (2022-10-08)
Revision as of 12:36, 8 October 2022 by Jianfei Song (Talk | contribs)

N terminal His-tagged sfGFP

This part contains a coding sequence of sfGFP (BBa_K515105) with N-terminal His tag from pET28a(+). It is intended to be used to characterize the crystal violet induction system in our project. The N-terminal His tag mimics the realistic protein expression condition of pET28a(+).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 64
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 217
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None