Part:BBa_K4180003

snf1Δ381-633aa C-truncated

SNF1Δ381-633aa, C-terminus truncated from amino acid 381 to 633 deleting autoinhibitory domain and SIP-interacting domain (SIR) in the SNF1 protein

Citation: McCartney, R R, and M C Schmidt. “Regulation of Snf1 kinase. Activation requires phosphorylation of threonine 210 by an upstream kinase as well as a distinct step mediated by the Snf4 subunit.” The Journal of biological chemistry vol. 276,39 (2001): 36460-6. doi:10.1074/jbc.M104418200>

Sequence and Features

;      BBa K4180003 snf1Δ381-633aa
     PCR product on lane 3 compared to
      BBa K4180002 snf1Δ2-306aa
        PCR product on lanes 1 and 2

After Bacteria transformation, bacteria colonies were picking up directly to do snf1Δ381-633aa PCR - colonies#1, 2, and 3 had BBa_K4180003 snf1Δ381-633aa. PCR products; lane 4 is yeast genomic DNA as template to do snf1Δ381-633aa PCR as a          comparison

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 528
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]