Composite

Part:BBa_K4345000:Design

Designed by: Luka Van den Berghe   Group: iGEM22_KU_Leuven   (2022-09-27)
Revision as of 09:43, 8 October 2022 by LVdB (Talk | contribs) (→‎References)


NarX fused to mNeonGreen with a rigid linker


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 659
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 659
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 260
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 659
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 659
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2236


Design Notes

NarX and mNeonGreen are fused with a rigid linker. This allows for an independent folding of both proteins.


Source

NarX is derived from E. coli genome, mNeonGreen is derived from Branchiostoma lanceolatum.

NarX sequence was obtained from NCBI and the sequence of mNeonGreen from the iGEM Registry of Standard Biological Parts.

References

narX sensor histidine kinase NarX [ Escherichia coli str. K-12 substr. MG1655 ]. (2022, September 22). National Library of Medicine - National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/gene/945788

Cavicchioli, R., Schröder, I., Schröder, S., Constanti, M., & Gunsalus, R. P. (1995). The NarX and NarQ Sensor-Transmitter Proteins of Escherichia coli Each Require Two Conserved Histidines for Nitrate-Dependent Signal Transduction to NarL. JOURNAL OF BACTERIOLOGY, 177(9), 2416–2424.

Cheung, J., & Hendrickson, W. A. (2009). Structural Analysis of Ligand Stimulation of the Histidine Kinase NarX. Structure, 17(2), 190–201. https://doi.org/10.1016/J.STR.2008.12.013