Coding

Part:BBa_K4144010

Designed by: Boshuo Zhou   Group: iGEM22_WHU-China   (2022-10-06)
Revision as of 20:13, 11 October 2022 by Peiqi (Talk | contribs)


Improved recombined SAMe transporter opPet8p fused with linker

Usage and Biology

Linkers can be classified into three groups: flexible, rigid and cleavable. Flexible linkers are generally composed of small, non-polar or polar residues such as Gly, Ser and Thr. The most common is the (Gly4Ser)n linker (Gly–Gly–Gly–Gly–Ser)n, where n indicates the number of repeats of the motif. Polyglycine linkers have also been evaluated, but the addition of a polar residue such as serine can reduce linker–protein interactions and preserve protein function. Due to their flexibility, these linkers are unstructured and thus provided limited domain separation in a previous study.

We WHU-China plan to use such linker to contact opPet8p and sfGFP in order to avoid the opPet8p membrane localization disruption by adding a sfGFP tag.

Figure. 1 Linker addition design into opPet8p::sfGFP recombined protein

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 520
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 390
    Illegal BamHI site found at 1018
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1642
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None