Part:BBa_K228004
NahR( reverse) - salicylate promoter
Designed by Lin Min Group: iGEM09_PKU_Beijing (2009-09-18)
Input: Salicylate molecules
Output: GFP fluorescence
| Part Main Page | Transfer Function | Growth Rate |
'Description=
This is a salicylate inducible promoter part constructed by Lin Min. The activator protein (NahR) gene is in the upstream but in an opposite direction of the regulated promoter. In the presence of salicylate, the promoter can be activated. If we place a generator on its downstream, the transcription of the coding sequence will be triggered by the salicylate-binded positive transcriptional activator protein which is encoded by NahR.
It is necessary to notice that there is no terminator downstream of the NahR coding sequence, but it has no effect son parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unexpected expression.
For the purpose of characterization, we connected BBa_E0840 downstream of the salicylate promoter to make the composite part, BBa_K228850. This report system allowed us to test the salicylate inducible promoter indirectly via the fluorescence output of BBa_E0840, while the input is salicylate solution at a gradient of concentration.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 408
- 1000COMPATIBLE WITH RFC[1000]
| chassis | shown to work in DH5α |
| copies | ~5 |
| input_s | salicylate molecules |
| protein | NahR |
| signalling_molecule | salicylate molecules |
| switch_point | 3.32μM for inducing of 2 hrs |