Composite

Part:BBa_K4137007:Design

Designed by: Andrea Huang   Group: iGEM22_GEMS_Taiwan   (2022-08-19)
Revision as of 03:20, 8 October 2022 by AnneDYK (Talk | contribs)


pLac+RBS+CcdB-Myc+B1006


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 271


Design Notes

RBS is used to enhance ccdB synthesis. pLac promoter is chosen for constitutive expression of ccdB. The fusion of ccdB and myc epitope tag allows the isolation of ccdB from the ccdB-ccdA complex upon purification for easy identification of individual concentrations.



Source

https://www.ncbi.nlm.nih.gov/nuccore/U51588.1 https://sci-hub.hkvisa.net/10.1016/0022-2836(85)90070-1 https://2018.igem.org/Team:Pasteur_Paris/Kill

References