Composite
Part:BBa_K4137008
Designed by: YA-JHIH KO Group: iGEM22_GEMS_Taiwan (2022-08-20)
Revision as of 04:43, 9 October 2022 by Andreahuang (Talk | contribs)
mleR expressing construct
This construct contains a T7 promoter, a RBS, a mleR coding complex, a mleR purification tag—6xHis, and a terminator B1006. This construct produce and purifies mleR, the regulator of CcdA, which produces when malate acid is presented.
Construct Designs
We attached a 6x His-Tag downstream of the mleR sequence for purification purposes. A T7 and RBS are attached upstream to the side of the open reading frame. The terminator BBa_B1006 is attached downstream of the sequence.
Characterization
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 49
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |