Composite
Part:BBa_K4137009
Designed by: Andrea Huang Group: iGEM22_GEMS_Taiwan (2022-10-07)
Revision as of 02:38, 8 October 2022 by Andreahuang (Talk | contribs)
T7+RBS+YebF-GS Linker-chi18h8-6xHis+B1006
This part consists of a T7 strong promoter, RBS (CTCCTT), a motor protein sequence yebF (BBa_K2922002), a GS Linker (BBa_J18921), a Chi19H8 coding sequence, a 6xHis tag (GTG x6), and a terminator (BBa_B1006). This part synthesizes and secretes a novel bacterial chitinase Chi18H8, which can be released extracellularly through e.coli Sec pathway (SecB-SecA-SecYEG, OmpF). This system continuously promotes the overexpression of Chi18H8 to lyse the chitin cell wall of the fungus fusarium oxysporum f.sp cubense, inhibiting fungal proliferation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1523
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1036
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 583
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |