Regulatory

Part:BBa_K4180001

Designed by: HuiChing Kuo   Group: iGEM22_KCIS_Xiugang_Taipei   (2022-07-09)
Revision as of 17:34, 7 October 2022 by TedChueh (Talk | contribs)

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pGal promoter

Gal promoter is in the plasmid and genes are cloned downstream of the pGal promoter, so the cloned genes can be induced in the presence of galactose
Galactose promoter can be induced in the presence of galactose and suppressed in the presence of glucose

Citation: Investigations in Molecular Cell Biology (O'Connor); 13.1: Regulation of the GAL1 promoter; <https://bio.libretexts.org/Bookshelves/Cell_and_Molecular_Biology/Book%3A_Investigations_in_Molecular_Cell_Biology_(O'Connor)/13%3A_Protein_overexpression/13.01%3A_Regulation_of_the_GAL1_promoter>

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 286
  • 1000
    COMPATIBLE WITH RFC[1000]

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Categories
Parameters
None