Part:BBa_K4180003

snf1Δ381-633aa C-truncated

SNF1Δ381-633aa, C-terminus truncated from amino acid 381 to 633 deleting autoinhibitory domain and SIP-interacting domain (SIR) in the SNF1 protein

Citation: Yang, T T et al. “Improved fluorescence and dual color detection with enhanced blue and green variants of the green fluorescent protein.” The Journal of biological chemistry vol. 273,14 (1998): 8212-6. doi:10.1074/jbc.273.14.8212

Sequence and Features

       BBa K4180003 snf1Δ381-633aa
      PCR product on lane 3 compared to
       BBa K4180002 snf1Δ2-306aa
        PCR product on lanes 1 and 2

        After Bacteria transformation, bacteria colonies were picking up directly to do snf1Δ381-633aa PCR - colonies#1, 2, and 3 had BBa_K4180003 snf1Δ381-633aa
         PCR products; lane 4 is yeast genomic DNA as template to do snf1Δ381-633aa PCR as a comparison

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 528
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]