Coding

Part:BBa_K4345016

Designed by: Paula Ventura   Group: iGEM22_KU_Leuven   (2022-09-30)
Revision as of 13:46, 8 October 2022 by LVdB (Talk | contribs)


ccdB with STOP codon control

CcdB protein is toxin of type II toxin-antitoxin system CcdB/CcdA. CcdB is a toxin that kills E. coli by targeting the GyrA subunit of DNA gyrase, an essential type II topoisomerase (Bahassi et al., 1999). In this part, we added three stop codons to prevent the translation and be able to clone CcdB into our host.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 249
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None