Composite

Part:BBa_K4156118

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-07)
Revision as of 07:33, 9 October 2022 by Liwei (Talk | contribs)


XOR gate-mRFP

XOR gate-mRFP consists of an XOR logic gate ( BBa_K4156117 ) and a downstream RFP sequence to test the performance of the XOR gate by detecting the red fluorescent signal.


Usage and Biology

We introduced a dual vector system in the same strain. The XOR gate-mRFP is located on pSB4C5, while its upstream promoter and corresponding TP901/BxB1 integrase are located on another plasmid, pSB1A3, which are resistant to chloramphenicol and ampicillin, respectively. Through the expression of red fluorescent protein in the dual vector system, we can monitor the change of red fluorescent signal of the recombinant vector to test the performance of XOR gate.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 104
    Illegal AgeI site found at 1097
    Illegal AgeI site found at 1209
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 124
    Illegal BsaI.rc site found at 351


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Categories
Parameters
None