Part:BBa_K4149086
The plasmid was modified by integrating the cyclopropane fatty acid (cfa )gene with pRSFDuet1 as a v
pRSFDuet1 is an E. coli protein dual expression vector, and we used this as a vector without T7 promoter in order to serve as a control group.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1492
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1492
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1492
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1492
Illegal NgoMIV site found at 1915 - 1000COMPATIBLE WITH RFC[1000]
Description: Compared with the control group at pH=5.0, the concentration of BL21-PRSF-1-cfa continued to increase higher than that of the control group after 8 hours. Under the condition of pH=7.0, the growth curve of BL21-PRSF-1-cfa strains basically coincided with that of the control group. We speculated that the overexpression of exogenous gene Accfa brought certain metabolic burden to the strains, which resulted in the failure of the recombinant strains to show the expected growth stability under mild environment. By comparing the differences of cyclopropane fatty acid content in cell membranes at different growth stages in different pH environments, we found that the difference of cyclopropane fatty acid content between the experimental group and the control group in the logarithmic phase was significantly higher than that in the stable phase. This may be related to the regulation of transcription level. We infer that this result is due to the orthogonality between σ subunits.
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