Composite

Part:BBa_K4156103

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-07)
Revision as of 06:24, 9 October 2022 by Liwei (Talk | contribs)


pLldR-Bxb1-φ174E

pLldR-Bxb1 is constructed with L-lactate-sensitive promoter pLldR and serine integrase Bxb1, and is uesd for lactate-specific response for logic gate coupling.


Usage and Biology

pLldR-Bxb1 consists of a fusion of the lactate-sensitive promoter pLldR and the serine integrase Bxb1. It will act as a complex regulatory for controlling downstream logic gates and transcription of genes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1479
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1753
    Illegal XhoI site found at 1840
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 614
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2587


[edit]
Categories
Parameters
None