Part:BBa_K4156099

pCadC-TP901

pCadC-TP901 is constructed with pH-sensitive promoter pCadC and serine integrase TP901.

Usage and Biology

pCadC-TP901 consists of a fusion of the pH-sensitive promoter pCadC and the serine integrase TP901. It will act as a complex regulatory for controlling downstream logic gates and transcription of genes.

In vitro characterization and data analysis of the reported strains

To improve signaling stability as well as accuracy, we added Amplifying genetic switches based on serine integrase (Bxb1,TP901) to the R reporter(BBa_) to construct the AR reporter. Fig 1 indicates pH (pCadc) induced AR reporters with homogenized fluorescence intensity (mRFP/Cell). In contrast to Fig 1 and 2, the fluorescence intensity of the AR reporter appeared more stable over time at pH 7.3 and was higher than that of the R reporter at pH 5.8, 6.3, and 7.3. This result indicates that the addition of amplifying genetic switch enhances the reporter intensity and robustness of the lactate biosensor.

Figure 1: Induction of downstream gene mRFP expression over time by the AR reporter consisting of pCadC+Switch+mRFP at different pH values.

Figure 2: Induction of downstream gene mRFP expression over time by the AR reporter consisting of pCadC +mRFP at different pH values.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1632
  
• 1000
  COMPATIBLE WITH RFC[1000]