Tag
Part:BBa_K4156096
Designed by: Zheng Huang Group: iGEM22_LZU-CHINA (2022-10-07)
SsrA tag (ANH)
A metabolically stable tmRNA in bacteria.
Usage and Biology
It has a unique structure with an alanyl tRNA-like structure at its 5′ and 3′ terminal sequences and an intermediate sequence encoding a tag peptide (tag peptide). In line with its unique structure, tmRNA has both tRNA and mRNA functions and participates in a specific translation reaction in cells, trans-translation, resulting in a chimeric protein with a tag peptide attached to the C-terminus.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |