DNA
Part:BBa_K4156094
Designed by: Zheng Huang Group: iGEM22_LZU-CHINA (2022-10-07)
CAP binding site
The site that binds specifically to CAP is a sequence that partially overlaps with Plac at the upstream end of the promoter P1ac of the 1ac manipulator element
Usage and Biology
CAP is formed by the combination of CRP and cAMP.When CAP binds to this sequence, it enhances the transcriptional activity of RNA polymerase, resulting in a 50-fold increase in transcription. In contrast, when glucose is available for catabolism, cAMP concentration decreases, CRP cannot be activated, and structural gene expression of the 1ac manipulator element decreases.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |