Part:BBa_K4385019
Copper-inducible surface display
This part is composed of CusSR two-component signal transport system, PcusC and OmpC-CBP.
Usage and Biology
Promoter PCusC can express downstream genes under the induction of Cu2+. When the zinc ion concentration in the environment exceeds a certain concentration, the PcusC promotes the transcription of downstream OmpC-CBP, and realizes the adsorption of zinc ions in the environment. The 6*His Tag was added to this sequence to purify the recombinant protein.
Characterization
Therefore, PCusC-OmpC-CBP-ST was constructed so that the OmpC-CBP would be expressed only the presence of copper ions in the environment.
To verify the construction of the PCusC-OmpC-CBP-ST, the enzyme digestion and agarose gel electrophoresis were performed (Fig. 2A). After 4 hrs of induction with 0.5, 1.0, 2.0 mM CuSO4 respectively, the concentration of Cu2+ in the supernatant was measured. The result also shows that our engineered bacteria have better capacity of copper absorption (Fig. 2B)
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 535
Illegal XbaI site found at 28
Illegal SpeI site found at 694
Illegal PstI site found at 1042 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 535
Illegal NheI site found at 1410
Illegal NheI site found at 1433
Illegal SpeI site found at 694
Illegal PstI site found at 1042 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 535
Illegal BglII site found at 243
Illegal BglII site found at 1545 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 535
Illegal XbaI site found at 28
Illegal SpeI site found at 694
Illegal PstI site found at 1042 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 535
Illegal XbaI site found at 28
Illegal SpeI site found at 694
Illegal PstI site found at 1042
Illegal AgeI site found at 361 - 1000COMPATIBLE WITH RFC[1000]
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