Regulatory

Part:BBa_K4140003

Designed by: Ahmed Gamal Mohamed Mattar   Group: iGEM22_AFCM-Egypt   (2022-09-24)
Revision as of 09:16, 7 October 2022 by Ahmed Mattar (Talk | contribs) (→‎Usage)


ParoF promoter


Part Description

ParoF promoter is a constitutive promoter present in E. Coli which is sensitive to tyrosine and regulated by tyrR protein, it intitiates the synthesis of DAHP (3-deoxy-D-arabino-heptulosonate 7-phosphate synthase) and it is what makes it sensitive to phenylalanine as TyrR & Phenylalanine complex causes inhibition of the transcription of aroF

Usage

Due to its sensitivity to TyrR we use this promoter to regulate the expression of L7Ae under the effect of tyrR and phenylalanine the activity of paroF is optimum for L7Ae expression but in the presence of TyrR and tyrosine the expression of L7Ae is markedly reduced as shown in figure 1.

Figure 1. (shows usage of Parof in our circuit design)





References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None