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Part:BBa_K4245000:Design

Designed by: Pablo Fernandez-Millan, Alexis Autour, Eric Ennifar, Eric Westhof, Michael Ryckelynck   Group: iGEM22_Lambert_GA   (2022-08-17)
Revision as of 19:58, 6 October 2022 by NickL (Talk | contribs) (→‎Design Notes)


iSpinach-D5-G30-A32 Aptamer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The iSpinach aptamer was originally generated by the researchers at the University of Strasbourg using the SELEX (systematic evolution of ligands by exponential enrichment) process (Millan et. al, 2017).

Source

The part was acquired as synthesized sequences from IDT and Twist Bioscience.

References

Fernandez-Millan, P., Autour, A., Ennifar, E., Westhof, E., & Ryckelynck, M. (2017). Crystal structure and fluorescence properties of the iSpinach aptamer in complex with DFHBI. RNA, 23(12), 1788–1795. https://doi.org/10.1261/rna.063008.117

Neubacher, S., & Hennig, S. (2018). RNA structure and cellular applications of fluorescent light-up Aptamers. Angewandte Chemie International Edition, 58(5), 1266–1279. https://doi.org/10.1002/anie.201806482

Paige, J. S., Wu, K. Y., & Jaffrey, S. R. (2011). RNA mimics of green fluorescent protein. Science, 333(6042), 642–646. https://doi.org/10.1126/science.1207339