Part:BBa_K4140025

Regulatory device

Part Description

Coding sequence for an enzyme that causes phenylalanine to be hydroxylated in order to produce tyrosine. PAH is a hydroxylase that needs biopterin. It's linked to KP-SP ISA secreting peptide signal at the N-terminus of the protein to transmit it from intra cellular to extra cellula

Usage

We are developing a tunable therapeutic approach to give the body the ability to produce phenylalanine hydroxylase (PAH) by implementing our designed therapeutic circuit in a cell-based system that produces PAH according to phenylalanine and tyrosine levels as shown in figure 1. In the presence of phenylalanine (Phe), the TyrR will activate tyrP, accordingly tyrP will enhance the production of PAH gene. While the inhibitory effect of the ParoF will not be initiated, thus L7Ae will be expressed, preventing the expression of the dcas12g.

Figure 1. (shows the whole cell-based biosensor.) Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 820
  Illegal PstI site found at 1294
  Illegal PstI site found at 2236
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 820
  Illegal PstI site found at 1294
  Illegal PstI site found at 2236
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 1815
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 820
  Illegal PstI site found at 1294
  Illegal PstI site found at 2236
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 820
  Illegal PstI site found at 1294
  Illegal PstI site found at 2236
  Illegal NgoMIV site found at 1737
  
• 1000
  COMPATIBLE WITH RFC[1000]