Composite

Part:BBa_K4140025

Designed by: Ahmed Gamal Mohamed Mattar   Group: iGEM22_AFCM-Egypt   (2022-09-28)
Revision as of 17:39, 6 October 2022 by Ahmed Mattar (Talk | contribs) (→‎Usage)


Regulatory device

Part Description

Coding sequence for an enzyme that causes phenylalanine to be hydroxylated in order to produce tyrosine. PAH is a hydroxylase that needs biopterin. It's linked to KP-SP ISA secreting peptide signal at the N-terminus of the protein to transmit it from intra cellular to extra cellula

Usage

We are developing a tunable therapeutic approach to give the body the ability to produce phenylalanine hydroxylase (PAH) by implementing our designed therapeutic circuit in a cell-based system that produces PAH according to phenylalanine and tyrosine levels as shown in figure 1. Figure 1. (shows the whole cell-based biosensor in our therapeutic approach. )

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 820
    Illegal PstI site found at 1294
    Illegal PstI site found at 2236
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 820
    Illegal PstI site found at 1294
    Illegal PstI site found at 2236
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1815
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 820
    Illegal PstI site found at 1294
    Illegal PstI site found at 2236
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 820
    Illegal PstI site found at 1294
    Illegal PstI site found at 2236
    Illegal NgoMIV site found at 1737
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None