Composite

Part:BBa_K4385020:Design

Designed by: Min An Zhao   Group: iGEM22_Jilin_China   (2022-10-06)
Revision as of 09:43, 7 October 2022 by Minan (Talk | contribs) (→‎Design Notes)

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Zinc-inducible surface display


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 616
    Illegal SpeI site found at 775
    Illegal PstI site found at 1123
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 616
    Illegal NheI site found at 1506
    Illegal NheI site found at 1529
    Illegal SpeI site found at 775
    Illegal PstI site found at 1123
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 616
    Illegal BglII site found at 324
    Illegal BglII site found at 1641
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 616
    Illegal SpeI site found at 775
    Illegal PstI site found at 1123
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 616
    Illegal SpeI site found at 775
    Illegal PstI site found at 1123
    Illegal AgeI site found at 442
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is mainly composed of zinc-inducible promoter(BBa_K4385007) , the anchoring protein OmpC-ZBP(BBa_K4385003, BBa_K4385004) and the heavy metal detoxification protein( BBa_K4385010).

Source

The basic parts that make up the composite part are all derived from E. coli genomic DNA.

References