Composite
Part:BBa_K4385015:Design
Designed by: Min An Zhao Group: iGEM22_Jilin_China (2022-10-04)
Copper constitutive surface display
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 414
Illegal SpeI site found at 573
Illegal PstI site found at 921 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 414
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1289
Illegal NheI site found at 1312
Illegal SpeI site found at 573
Illegal PstI site found at 921 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 414
Illegal BglII site found at 122
Illegal BglII site found at 1424 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 414
Illegal SpeI site found at 573
Illegal PstI site found at 921 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 414
Illegal SpeI site found at 573
Illegal PstI site found at 921
Illegal AgeI site found at 240 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
It is mainly composed of constitutive promoter(BBa_J23100) , the anchoring protein OmpC-CBP(BBa_K4385003, BBa_K4385005) and Heavy metal detoxification protein( BBa_K4385010).
Source
The basic parts that make up the composite part are all derived from E. coli genomic DNA.