Protein_Domain
Part:BBa_K4137011
Designed by: YA-JHIH KO Group: iGEM22_GEMS_Taiwan (2022-10-06)
CcdB coding sequence + 6xHis
The above sequence encodes for the CcdB protein, a component of the CcdA-CcdB toxin-antitoxin system. CcdB binds with CcdA which inhibits CcdB from targeting DNA gyrase thus preventing DNA double-strand breaks. The 6xHis tag is used for protein purification and identification.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 234
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Categories
Parameters
None |