Composite
PcusC-GFP

Part:BBa_K4385011

Designed by: Min An Zhao   Group: iGEM22_Jilin_China   (2022-10-01)
Revision as of 14:09, 6 October 2022 by Minan (Talk | contribs)


Lead sensor

The lead sensor is composed of lead-inducible promoter and GFP. When there is no lead ion in the environment, PbrR protein is expressed in the presence of Pbr constitutive promoter and binds to its downstream inducible promoter Pbc to repress GFP expression; when lead ion reaches a certain concentration in the environment, PbrR protein chelates lead ion so that it cannot bind to the inducible promoter Pbc and downstream GFP can be expressed and the engineered bacteria can see green fluorescence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 462
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 462
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 462
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 462
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 253
    Illegal BsaI.rc site found at 1394


[edit]
Categories
Parameters
None