Part:BBa_K4165142

MMD3rev Peptide

Tau binding peptide targeting the PHF seed of Tau

Usage and Biology

MMD3rev peptide sequence is YWVSTHRAKLPD, it binds to 275-VQIINK-280 (PHF*) and 306-VQIVYK-311 (PHF) motifs of tau that are reported to drive tau paired helical filament aggregates. it inhibits formation of PHF6* and full‐length Tau aggregates, but not PHF driven aggregates, and prevents the formation of tau β‐sheet‐rich fibrils.

Dry Lab

Modeling

MMD3rev is modeled by AlphaFold2, Apptest, ITASSER and TrRosetta, best model obtained from ITASSER.

                            Figure 1.: Predicted 3D structure of Synthetic peptide MMD3rev.

Table 1: Quality assessment parameters of MMD3rev model.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Malhis, M., Kaniyappan, S., Aillaud, I., Chandupatla, R. R., Ramirez, L. M., Zweckstetter, M., ... & Funke, S. A. (2021). Potent Tau Aggregation Inhibitor D‐Peptides Selected against Tau‐Repeat 2 Using Mirror Image Phage Display. ChemBioChem, 22(21), 3049-3059.