Coding
ccdB-LVA

Part:BBa_K176157

Designed by: Danqian Liu, Zongxiao He, Hao Jiang   Group: iGEM09_USTC   (2009-09-14)
Revision as of 17:35, 9 July 2015 by Vinoo (Talk | contribs)

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ccdB-LVA coding sequence

All ccdb parts have been withdrawn from the Registry. Samples of parts containing the ccdB gene cannot be requested. - iGEM HQ

Add LVA degradation tag (widely used in C0040, C0051, C0061...) to ccdB(K145151) C-terminal: 

[K145151 without stop codon] GCT GCA AAC GAC GAA AAC TAC GCT TTA GTA GCT [TAA TAA]
                              A   A   N   D   E   N   Y   A   L   V   A

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 216


[edit]
Categories
Parameters
None