Part:BBa_K4165192

Amyloid beta peptide 12 (Aβ 29-42)

This part encodes a part of the Amyloid 𝛽 fragment (29-42) which has the ability to bind to A𝛽 plaques inside the brain.

Usage and Biology

Type of Amyloid-beta binding peptide start from amino acid 32 to 42, this peptide characterized by its solubility which is 22 ± 9 μM, highly toxic effect

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Features and codon optimize

This sequence is optimized for E. coli bacteria

Source

Homo sapiens Amyloid-beta precursor protein - UniProt (P05067)

References

Zhao, Y., Cai, J., Liu, Z., Li, Y., Zheng, C., Zheng, Y., ... & Liu, Y. (2018). Nanocomposites inhibit the formation, mitigate the neurotoxicity, and facilitate the removal of β-amyloid aggregates in Alzheimer’s disease mice. Nano letters, 19(2), 674-683.

Dry lab

Modeling

Due to the nonavailability of a model to this peptide we modeled it in our used modeling software's and after the Running of the quality assessment, the models have been scored to get the top model.

                              Figure 1.: Amyloid-beta (29-42) top model visualized by Pymol.