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Part:BBa_J70500:Design

Designed by: Tom Knight   Group: Knight Lab   (2009-09-13)
Revision as of 22:15, 13 September 2009 by Tk (Talk | contribs) (→‎Design Notes)

pWV01 broad host range plasmid origin and rep protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Avoided a single Sau3AI site near the claI site, to eliminate Sau3AI sites in the final plasmid assembly.

  • PCR with primers:
    • WV-F: gtttcttcgaattcgcggccgcttctagagcgattttttattaaaacgtctcaaaatcgtttctg
    • WV-R: gtttcttcctgcagcggccgctactagtaatcattttgtttattgcaattgtattgctattaatcg

These primers were designed with Austin's GBW tool.

Source

PCR of the plasmid pMG, kind gift from Prof. Jan Kok. This plasmid contains the ClaI fragment of the pWV01 plasmid.

References