Reporter
BFP

Part:BBa_K4321001

Designed by: Kulay Janneh   Group: iGEM22_Guelph   (2022-09-28)
Revision as of 17:46, 5 October 2022 by Kjanneh (Talk | contribs)


BFP

This blue fluorescent protein (BFP) one of the 19 variants of Aequorea victoria derived green fluorescent protein (GFP).

Usage and Biology

This BFP differs from its predecessor in that it contains a tyrosine (Y) to histidine (H) substitution at position 66. This Y66H substitution results in the board excitation close to 380 namometers and emission at 448 namometers. This BFP variant carries an additional modification, the addition of a mammalian codon directly downstream of the start methionin allowing its expression in mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter
//function/reporter/fluorescence
Parameters
color