Composite

Part:BBa_K4179010:Design

Designed by: Yasmin Habib   Group: iGEM22_Technion-Israel   (2022-10-05)
Revision as of 16:33, 5 October 2022 by Yasmin Habib (Talk | contribs) (→‎Design Notes)

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UDT under Rhlr promoter + P2A + mCherry reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2134
    Illegal PstI site found at 1774
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1339
    Illegal PstI site found at 1774
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2134
    Illegal PstI site found at 1774
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2134
    Illegal PstI site found at 1774
    Illegal AgeI site found at 1413
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The linker (BBa_K4179004) contains a 6xHis tag and a thrombin recognition and cleavage site.