Plasmid_Backbone

Part:BBa_K4166004

Designed by: George Amoako   Group: iGEM22_Alma   (2022-10-04)
Revision as of 02:47, 12 October 2022 by Djcamenares (Talk | contribs)


Camp +1 mutation.

Increased plasmid copy number. Increased P2 extension, stabilizes secondary structure in promoter.

Plasmid Copy Number and Expression - Alma 2022

We created a series of mutants of BBa_J04450 in the origin of replication, and wanted to both quantify the copy number and see the effect the copy number has on gene expression.

T--Alma--pcnPlate.png

We found that, although this mutation increases plasmid copy number, it actually decreases expression. It could be due to collisions between DNA polymerase (attempting to replicate the plasmid) and RNA polymerase (attempting to transcribe the RFP gene).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


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Categories
Parameters
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