Coding

Part:BBa_K4323002

Designed by: Jessica Marinelli, Kevin Lim, Chun Hin Wong   Group: iGEM22_UManitoba   (2022-09-30)
Revision as of 22:26, 4 October 2022 by Prefont5 (Talk | contribs)


Truncated FitD

This part is a truncated version of FitD, a molluscicide from Pseudomonas protegens. It is a toxin that can be used to kill quagga and zebra mussels. This part is a coding sequence with a polyhistidine tag for purification. This part is codon optimized for expression in Escherichia coli. This part is a different form of FitD in comparison to BBa_K2683006.

<img src="fitd-toxicity.png">



Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 909
    Illegal BamHI site found at 1019
    Illegal BamHI site found at 2568
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2191
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None