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Coding
Part:BBa_K4268000:Experience
Designed by: J. Aubrey, J. Alvarenga, L. Buchanan, J. Reyes, D. Yashinski Group: iGEM22_SUNY_Oneonta (2022-07-15)
Revision as of 03:17, 7 October 2022 by Jazmine124 (Talk | contribs)
The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.
The gel indicates that all five colonies are likely to contain the correct insert and thus, the Head-Tail Connector was successfully cloned into a Level O Golden Gate Assembly basic part.
Applications of BBa_K4268000
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UNIQf528173fd4bf77fd-partinfo-00000000-QINU UNIQf528173fd4bf77fd-partinfo-00000001-QINU