Regulatory

Part:BBa_K4115012:Design

Designed by: Shenxi Cao   Group: iGEM22_ShanghaiTech_China   (2022-09-21)
Revision as of 12:35, 4 October 2022 by ParadoxDecade (Talk | contribs) (→‎Design Notes)

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lac UV5 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 82
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 82
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 82
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The lac regulatory system represented by it can also be used in Azorhizobium caulinodans ORS571 strain. This regulatory part can be used by almost all prokaryotes throughout the project.

Source

The lac UV5 promoter sequence is from the E. coli genome.

References