Composite

Part:BBa_K4115041:Design

Designed by: Kaijun Wang   Group: iGEM22_ShanghaiTech_China   (2022-09-30)
Revision as of 08:16, 4 October 2022 by ParadoxDecade (Talk | contribs) (→‎References)

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nifA_upstream-BleoR-nifA_downstream


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 434
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1427
    Illegal BamHI site found at 1766
    Illegal XhoI site found at 547
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 234
    Illegal NgoMIV site found at 339
    Illegal NgoMIV site found at 556
    Illegal NgoMIV site found at 1179
    Illegal NgoMIV site found at 1240
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 331
    Illegal BsaI.rc site found at 1287


Design Notes

Add the upstream and downstream fragments of the nitrogen-fixing bacteria (A. caul) nifA gene upstream and downstream of the bleomycin resistance gene. This component was used in the project to edit the nitrogen-fixing bacteria (A. caul) genome by genetic recombination methods.[1]

Source

Build via Gibson Assembly.

References

[1]Ryu, M. H., Zhang, J., Toth, T., Khokhani, D., Geddes, B. A., Mus, F., Garcia-Costas, A., Peters, J. W., Poole, P. S., Ané, J. M., & Voigt, C. A. (2020). Control of nitrogen fixation in bacteria that associate with cereals. Nature microbiology, 5(2), 314–330. https://doi.org/10.1038/s41564-019-0631-2