RNA

Part:BBa_K4115015:Design

Designed by: Renjie Yu   Group: iGEM22_ShanghaiTech_China   (2022-09-22)
Revision as of 03:41, 4 October 2022 by Yurj (Talk | contribs) (→‎Source)


glgA sRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of glgA sRNA is designed as the reverse complementary sequence of 25 base sequences starting from the SD sequences of glgA. And HFQ sequence should be designed in the same plasmid with this sRNA.[1]

Source

The glgA gene sequence is gained from the genome of the S.elongatus PCC 6803, and the fragments we use were artificially synthesized by the company.

References

[1] Li, S., Sun, T., Chen, L., Zhang, W. (2021). Designing and Constructing Artificial Small RNAs for Gene Regulation and Carbon Flux Redirection in Photosynthetic Cyanobacteria. In: Basu, C. (eds) Biofuels and Biodiesel. Methods in Molecular Biology, vol 2290. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1323-8_16