DNA
Part:BBa_K4115009
Designed by: Renjie Yu Group: iGEM22_ShanghaiTech_China (2022-09-21)
NS3-2
Neutral site 3(NS3) is commonly used in homologous recombination for S.elongatus, where NS3-1 acts as an upstream homology arm. [1]
Usage and Biology
It is used together with NS3-1 (BBa_K3228025) to construct plasmids required for S.elongatus HL7942, where NS3-2 is placed upstream of the aimed fragments.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 507
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 507
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 507
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 507
- 1000COMPATIBLE WITH RFC[1000]
References
[1] Niederholtmeyer H, Wolfstädter BT, Savage DF, Silver PA, Way JC. Engineering cyanobacteria to synthesize and export hydrophilic products. Appl Environ Microbiol. 2010 Jun;76(11):3462-6. doi: 10.1128/AEM.00202-10. Epub 2010 Apr 2. Erratum in: Appl Environ Microbiol. 2010 Sep;76(17):6023. PMID: 20363793; PMCID: PMC2876443.
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Categories
Parameters
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