DNA

Part:BBa_K4115009

Designed by: Renjie Yu   Group: iGEM22_ShanghaiTech_China   (2022-09-21)
Revision as of 02:34, 4 October 2022 by Yurj (Talk | contribs)


NS3-2

Neutral site 3(NS3) is commonly used in homologous recombination for S.elongatus, where NS3-1 acts as an upstream homology arm. [1]


Usage and Biology

It is used together with NS3-1 (BBa_K3228025) to construct plasmids required for S.elongatus HL7942, where NS3-2 is placed upstream of the aimed fragments.

References

[1] Niederholtmeyer H, Wolfstädter BT, Savage DF, Silver PA, Way JC. Engineering cyanobacteria to synthesize and export hydrophilic products. Appl Environ Microbiol. 2010 Jun;76(11):3462-6. doi: 10.1128/AEM.00202-10. Epub 2010 Apr 2. Erratum in: Appl Environ Microbiol. 2010 Sep;76(17):6023. PMID: 20363793; PMCID: PMC2876443.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 507
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 507
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 507
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 507
  • 1000
    COMPATIBLE WITH RFC[1000]

[edit]
Categories
Parameters
None