Composite

Part:BBa_K4115036

Designed by: Kaijun Wang   Group: iGEM22_ShanghaiTech_China   (2022-09-30)
Revision as of 13:15, 3 October 2022 by ParadoxDecade (Talk | contribs)


J23101-B0034-SacC-B0015
The J23101 promoter on this part constitutively expresses SacC protein in E. coli.


Usage and Biology

Constitutive expression of the SacC protein in E. coli is expected to confer the ability to efficiently utilize extracellular sucrose produced by cyanobacteria. See <parts>BBa_K4115017</parts> for further details.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1370
    Illegal PstI site found at 498
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 498
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 498
    Illegal NgoMIV site found at 757
    Illegal AgeI site found at 479
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None