![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4115017
Designed by: Shenxi Cao Group: iGEM22_ShanghaiTech_China (2022-09-23)
Revision as of 06:57, 3 October 2022 by ParadoxDecade (Talk | contribs)
SacC (the extracellular sucrase gene)
The sequence of Zymomonas mobilis gene sacC that encodes the extracellular sucrase. Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 437
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1309
Illegal PstI site found at 437 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1352
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 437
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 437
Illegal NgoMIV site found at 696
Illegal AgeI site found at 418 - 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
The expression product is exhibited sucrase but not levansucrase activity,which means it behaves like a true sucrase. Heterologous expression of SacC aims to improve the utilization of sucrose in the medium by E. coli responsible for the synthetic production of biologics.
![](/wiki/images/d/dc/SacCgrowthcurve1.png)
Figure 1: Fermentation of E. coli with BBa_K4115017 in 300ml conical flask in biological shaker, scale: 100 mL M9 medium, 37 °C, 220 rpm, initial pH 7.5, OD600 measured every 2 hours.
[edit]
Categories
Parameters
None |