DNA

Part:BBa_K4218015:Design

Designed by: Wuhui Jiang   Group: iGEM22_YiYe-China   (2022-10-03)
Revision as of 06:33, 3 October 2022 by CholeJiang (Talk | contribs) (→‎Source)

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ecessive exon of MAP3K7-mCherry


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 464
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 464
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 464
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 464
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

During normal splicing of the cell, the internally shifted cryptic exon will be removed, mCherry will be expressed. Because the stop codon was located in front of the GFP gene, GFP could not be translatable and red fluorescent could be detected in cells. In the case where splicing is inhibited, the internally shifted recessive exon will not be removed, the mCherry gene is shifted.


Source

Ecessive exon is form MAP3K7 gene.MCherry is a red fluorescent dye that is widely used in biotechnology as a tracer, ,which isolated from the Discosoma species.We added a A shifted in-frame termination codon (TGATG) at the end of the mcherry sequence.

References