Coding

Part:BBa_K200005:Design

Designed by: Royah Vaezi, David Roche   Group: iGEM09_Imperial College London   (2009-08-12)
Revision as of 13:25, 2 September 2009 by Droche (Talk | contribs) (→‎Design Notes)

OtsA: Part 1 of 2 for trehalose producing enzymes.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 383
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR from Escherichia Coli BL21(DE3) using Pfu Ultra II Polymerase.


Forward: GCTCTAGATGAGTCGTTTAGTCGTAG
Recomended Temperatures for PCR : 52.4C(without overhang) and 63.2(with overhang)

Backward: GGACTAGTACTACGCAAGCTTTGGAAAG
Recomended Temperatures for PCR : 54.5C(without overhang) and 65.1(with overhang)
BioBrick overhang shown in bold

Source

Escherichia coli BL21(DE3)

References